Human SIRT7(NAD-dependent deacetylase sirtuin-7) ELISA Kit

说明书
  • 规格
Product name

Human SIRT7(NAD-dependent deacetylase sirtuin-7) ELISA Kit

Catalogue No.

EH1296

Alias

SIRT7(NAD-dependent deacetylase sirtuin-7) ELISA Kit, SIR2L7 ELISA Kit, Regulatory protein SIR2 homolog 7 ELISA Kit, SIR2-like protein 7 ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

SIRT7 ELISA Kit allows for the in vitro quantitative determination of SIRT7 concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

0.312-20ng/ml

Sensitivity

< 0.188ng/ml

Standard curve

Human SIRT7(NAD-dependent deacetylase sirtuin-7) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of SIRT7 and the recovery rates were calculated by comparing the measured value to the expected amount of SIRT7 in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 90-104 95
EDTA plasma(n=5) 87-105 93
heparin plasma(n=5) 85-98 92
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of SIRT7 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 85-104% 93-102% 93-102% 85-103%
EDTA plasma(n=5) 86-97% 82-99% 84-98% 83-97%
heparin plasma(n=5) 80-100% 80-100% 85-96% 85-93%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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