Human SERPINF1(Pigment epithelium-derived factor) ELISA Kit

说明书
  • 规格
Product name

Human SERPINF1(Pigment epithelium-derived factor) ELISA Kit

Catalogue No.

EH1048

Alias

PEDF(Pigment Epithelium Derived Factor ) ELISA Kit, Serpin F1 ELISA Kit, Pigment epithelium-derived factor ELISA Kit, Cell proliferation-inducing gene 35 protein ELISA Kit, EPC-1 ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

SERPINF1 ELISA Kit allows for the in vitro quantitative determination of SERPINF1 concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

0.156-10ng/ml

Sensitivity

< 0.094ng/ml

Standard curve

Human SERPINF1(Pigment epithelium-derived factor) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of SERPINF1 and the recovery rates were calculated by comparing the measured value to the expected amount of SERPINF1 in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 92-102 97
EDTA plasma(n=5) 92-98 94
heparin plasma(n=5) 94-100 96
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of SERPINF1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 86-105% 85-101% 86-94% 88-105%
EDTA plasma(n=5) 89-99% 83-89% 83-100% 83-101%
heparin plasma(n=5) 90-96% 89-99% 80-100% 80-97%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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