Human PYGB(Glycogen phosphorylase, brain form) ELISA Kit

说明书
  • 规格
Product name

Human PYGB(Glycogen phosphorylase, brain form) ELISA Kit

Catalogue No.

EH1070

Alias

PYGB ELISA Kit, Glycogen phosphorylase brain form ELISA Kit, Glycogen Phosphorylase BB ELISA Kit, GPBB ELISA Kit, Brain glycogen phosphorylase ELISA Kit, Glycogen phosphorylase B ELISA Kit, Glycogen Phosphorylase Isoenzyme BB ELISA Kit, Phosphorylase glycogen brain ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

PYGB ELISA Kit allows for the in vitro quantitative determination of PYGB concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

15.6-1000pg/ml

Sensitivity

< 9.375pg/ml

Standard curve

Human PYGB(Glycogen phosphorylase, brain form) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of PYGB and the recovery rates were calculated by comparing the measured value to the expected amount of PYGB in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 87-100 95
EDTA plasma(n=5) 87-104 97
heparin plasma(n=5) 85-104 94
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PYGB and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 87-93% 86-105% 87-97% 94-105%
EDTA plasma(n=5) 88-99% 82-94% 82-101% 83-98%
heparin plasma(n=5) 85-97% 80-95% 90-100% 83-98%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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