Human PDGFB(Platelet-derived growth factor subunit B) ELISA Kit

说明书
  • 规格
Product name

Human PDGFB(Platelet-derived growth factor subunit B) ELISA Kit

Catalogue No.

EH0548

Alias

PDGFB ELISA Kit, B chain ELISA Kit, Becaplermin ELISA Kit, FLJ12858 ELISA Kit, PDGF subunit B ELISA Kit, PDGF2 ELISA Kit, PDGF-2 ELISA Kit, platelet-derived growth factor 2 ELISA Kit, Platelet-derived growth factor beta polypeptide ELISA Kit, platelet-derived growth factor subunit B ELISA Kit, platelet-derived growth factor, B chain ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

PDGFB ELISA Kit allows for the in vitro quantitative determination of PDGFB concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

23.438-1500pg/ml

Sensitivity

< 14.063pg/ml

Standard curve

Human PDGFB(Platelet-derived growth factor subunit B) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of PDGFB and the recovery rates were calculated by comparing the measured value to the expected amount of PDGFB in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 85-100 92
EDTA plasma(n=5) 85-102 94
heparin plasma(n=5) 87-102 93
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PDGFB and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 98-105% 87-98% 95-104% 89-102%
EDTA plasma(n=5) 82-98% 83-91% 89-96% 85-95%
heparin plasma(n=5) 86-95% 80-100% 83-95% 84-94%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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