Human PDE10A(cAMP and cAMP-inhibited cGMP 3′,5′-cyclic phosphodiesterase 10A) ELISA Kit

说明书
  • 规格
Product name

Human PDE10A(cAMP and cAMP-inhibited cGMP 3′,5′-cyclic phosphodiesterase 10A) ELISA Kit

Catalogue No.

EH1324

Alias

PDE10A ELISA Kit, cAMP and cAMP-inhibited cGMP 3′,5′-cyclic phosphodiesterase 10A ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

PDE10A ELISA Kit allows for the in vitro quantitative determination of PDE10A concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

0.312-20ng/ml

Sensitivity

< 0.188ng/ml

Standard curve

Human PDE10A(cAMP and cAMP-inhibited cGMP 3′,5′-cyclic phosphodiesterase 10A) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of PDE10A and the recovery rates were calculated by comparing the measured value to the expected amount of PDE10A in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 85-103 95
EDTA plasma(n=5) 92-104 98
heparin plasma(n=5) 92-104 99
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PDE10A and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 85-95% 91-103% 86-105% 88-98%
EDTA plasma(n=5) 86-99% 84-101% 84-98% 82-96%
heparin plasma(n=5) 83-96% 87-98% 80-98% 83-100%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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