Human P4HA2(Prolyl 4-hydroxylase subunit alpha-2) ELISA Kit

说明书
  • 规格
Product name

Human P4HA2(Prolyl 4-hydroxylase subunit alpha-2) ELISA Kit

Catalogue No.

EH1229

Alias

4-PH alpha-2(Prolyl 4-hydroxylase subunit alpha-2) ELISA Kit, P4HA2 ELISA Kit, UNQ290 ELISA Kit, PRO330 ELISA Kit, Procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-2 ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

P4HA2 ELISA Kit allows for the in vitro quantitative determination of P4HA2 concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

0.78-50ng/ml

Sensitivity

< 0.469ng/ml

Standard curve

Human P4HA2(Prolyl 4-hydroxylase subunit alpha-2) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of P4HA2 and the recovery rates were calculated by comparing the measured value to the expected amount of P4HA2 in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 86-105 93
EDTA plasma(n=5) 86-101 96
heparin plasma(n=5) 91-98 93
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of P4HA2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 91-104% 91-103% 86-105% 85-103%
EDTA plasma(n=5) 84-91% 82-95% 85-96% 86-98%
heparin plasma(n=5) 86-90% 85-95% 84-99% 83-97%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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