Human NOS3/eNOS(Nitric oxide synthase, endothelial) ELISA Kit

说明书
  • 规格
Product name

Human NOS3/eNOS(Nitric oxide synthase, endothelial) ELISA Kit

Catalogue No.

EH0554

Alias

NOS3 ELISA Kit, eNOS ELISA Kit, Constitutive NOS ELISA Kit, ECNOS ELISA Kit, endothelial ELISA Kit, Endothelial NOS ELISA Kit, eNOS ELISA Kit, cNOS ELISA Kit, nitric oxide synthase 3(endothelial cell) ELISA Kit, NOS type III ELISA Kit, NOSIII ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

NOS3/eNOS ELISA Kit allows for the in vitro quantitative determination of NOS3/eNOS concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

62.5-4000pg/ml

Sensitivity

< 37.5pg/ml

Standard curve

Human NOS3/eNOS(Nitric oxide synthase, endothelial) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of NOS3/eNOS and the recovery rates were calculated by comparing the measured value to the expected amount of NOS3/eNOS in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 87-105 94
EDTA plasma(n=5) 90-105 97
heparin plasma(n=5) 90-98 93
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NOS3/eNOS and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 92-102% 87-102% 92-103% 93-102%
EDTA plasma(n=5) 83-101% 88-100% 82-96% 83-98%
heparin plasma(n=5) 82-97% 80-100% 80-96% 90-99%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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