Human NAMPT(Nicotinamide phosphoribosyltransferase) ELISA Kit

说明书
  • 规格
Product name

Human NAMPT(Nicotinamide phosphoribosyltransferase) ELISA Kit

Catalogue No.

EH0651

Alias

VF(Visfatin) ELISA Kit, NAMPT ELISA Kit, NAmPRTase ELISA Kit, NMPRTase ELISA Kit, PBEF1 ELISA Kit, Visfatin ELISA Kit, NAmPRTase ELISA Kit, Nampt ELISA Kit, nicotinamide phosphoribosyltransferase ELISA Kit, Pre-B cell-enhancing factor ELISA Kit, pre-B-cell colony enhancing factor 1 ELISA Kit, Pre-B-cell colony-enhancing factor 1 ELISA Kit, VISFATIN ELISA Kit, Visfatin ELISA Kit, PBEF ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

NAMPT ELISA Kit allows for the in vitro quantitative determination of NAMPT concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

0.313-20ng/ml

Sensitivity

< 0.188ng/ml

Standard curve

Human NAMPT(Nicotinamide phosphoribosyltransferase) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of NAMPT and the recovery rates were calculated by comparing the measured value to the expected amount of NAMPT in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 88-102 96
EDTA plasma(n=5) 95-103 100
heparin plasma(n=5) 95-105 100
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NAMPT and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 87-105% 88-101% 89-100% 87-103%
EDTA plasma(n=5) 84-96% 83-101% 90-101% 83-100%
heparin plasma(n=5) 81-96% 81-90% 80-99% 80-99%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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