Human MMP-12(Macrophage metalloelastase) ELISA Kit

说明书
  • 规格
Product name

Human MMP-12(Macrophage metalloelastase) ELISA Kit

Catalogue No.

EH0233

Alias

MMP-12 ELISA Kit, MMP12 ELISA Kit, HME ELISA Kit, ME ELISA Kit, MME ELISA Kit, Macrophage Metalloelastase ELISA Kit, Macrophage Elastase ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

MMP-12 ELISA Kit allows for the in vitro quantitative determination of MMP-12 concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

0.313-20ng/ml

Sensitivity

< 0.188ng/ml

Standard curve

Human MMP-12(Macrophage metalloelastase) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of MMP-12 and the recovery rates were calculated by comparing the measured value to the expected amount of MMP-12 in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 89-98 93
EDTA plasma(n=5) 87-104 95
heparin plasma(n=5) 91-100 95
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MMP-12 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 92-105% 91-104% 86-103% 85-92%
EDTA plasma(n=5) 82-96% 84-93% 89-96% 88-98%
heparin plasma(n=5) 81-95% 81-97% 84-93% 84-100%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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