Human MIP-3a/CCL20(Macrophage Inflammatory Protein 3 Alpha) ELISA Kit

说明书
  • 规格
Product name

Human MIP-3a/CCL20(Macrophage Inflammatory Protein 3 Alpha) ELISA Kit

Catalogue No.

EH0231

Alias

MIP-3α ELISA Kit, MIP-3a ELISA Kit, CCL20 ELISA Kit, CKb4 ELISA Kit, LARC ELISA Kit, MIP3A ELISA Kit, SCYA20 ELISA Kit, ST38 ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

MIP-3a/CCL20 ELISA Kit allows for the in vitro quantitative determination of MIP-3a/CCL20 concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

15.625-1000pg/ml

Sensitivity

< 9.375pg/ml

Standard curve

Human MIP-3a/CCL20(Macrophage Inflammatory Protein 3 Alpha) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of MIP-3a/CCL20 and the recovery rates were calculated by comparing the measured value to the expected amount of MIP-3a/CCL20 in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 87-103 95
EDTA plasma(n=5) 86-100 92
heparin plasma(n=5) 88-104 98
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MIP-3a/CCL20 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 94-103% 92-104% 89-102% 91-102%
EDTA plasma(n=5) 83-101% 87-93% 91-97% 83-90%
heparin plasma(n=5) 84-97% 83-100% 85-99% 93-100%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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