Human MIP-1α(Macrophage Inflammatory Protein 1 Alpha) ELISA Kit

说明书
  • 规格
Product name

Human MIP-1α(Macrophage Inflammatory Protein 1 Alpha) ELISA Kit

Catalogue No.

EH0230

Alias

MIP-1α(Macrophage Inflammatory Protein 1 Alpha) ELISA Kit, CCL3 ELISA Kit, LD78ALPHA ELISA Kit, MIP-1-alpha ELISA Kit, MIP1A ELISA Kit, SCYA3 ELISA Kit, G0S19-1 ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

MIP-1α ELISA Kit allows for the in vitro quantitative determination of MIP-1α concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

23.438-1500pg/ml

Sensitivity

< 14.063pg/ml

Standard curve

Human MIP-1α(Macrophage Inflammatory Protein 1 Alpha) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of MIP-1α and the recovery rates were calculated by comparing the measured value to the expected amount of MIP-1α in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 87-95 92
EDTA plasma(n=5) 85-101 91
heparin plasma(n=5) 85-103 91
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MIP-1α and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 88-104% 95-102% 87-105% 86-104%
EDTA plasma(n=5) 83-100% 85-94% 86-100% 83-96%
heparin plasma(n=5) 80-97% 87-94% 85-100% 81-99%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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