Human MFGE8(Lactadherin) ELISA Kit

说明书
  • 规格
Product name

Human MFGE8(Lactadherin) ELISA Kit

Catalogue No.

EH0226

Alias

MFGE8 ELISA Kit, SED1 ELISA Kit, Milk fat globule-EGF factor 8(MFG-E8) ELISA Kit, MFGM ELISA Kit, Breast epithelial antigen BA46 ELISA Kit, HMFG ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

MFGE8 ELISA Kit allows for the in vitro quantitative determination of MFGE8 concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

31.2-2000pg/ml

Sensitivity

< 18.75pg/ml

Standard curve

Human MFGE8(Lactadherin) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of MFGE8 and the recovery rates were calculated by comparing the measured value to the expected amount of MFGE8 in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 90-105 98
EDTA plasma(n=5) 87-103 93
heparin plasma(n=5) 86-105 94
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MFGE8 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 94-104% 98-101% 87-101% 86-101%
EDTA plasma(n=5) 84-96% 84-94% 85-101% 83-98%
heparin plasma(n=5) 82-97% 81-98% 88-99% 82-100%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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