Human MAP2K5(Dual specificity mitogen-activated protein kinase kinase 5) ELISA Kit

说明书
  • 规格
Product name

Human MAP2K5(Dual specificity mitogen-activated protein kinase kinase 5) ELISA Kit

Catalogue No.

EH0572

Alias

MAP2K5 ELISA Kit, MEK5 ELISA Kit, MAPKK5 ELISA Kit, PRKMK5 ELISA Kit, HsT17454 ELISA Kit, MAPK kinase 5 ELISA Kit, ERK kinase 5 ELISA Kit, MAP kinase kinase 5 ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

MAP2K5 ELISA Kit allows for the in vitro quantitative determination of MAP2K5 concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

0.312-20ng/ml

Sensitivity

< 0.188ng/ml

Standard curve

Human MAP2K5(Dual specificity mitogen-activated protein kinase kinase 5) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of MAP2K5 and the recovery rates were calculated by comparing the measured value to the expected amount of MAP2K5 in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 90-102 96
EDTA plasma(n=5) 85-96 92
heparin plasma(n=5) 87-103 94
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MAP2K5 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 88-102% 88-105% 85-103% 95-103%
EDTA plasma(n=5) 83-92% 86-96% 89-101% 82-99%
heparin plasma(n=5) 85-96% 83-92% 86-100% 82-98%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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