Human IL-5(Interleukin 5) ELISA Kit

说明书
  • 规格
Product name

Human IL-5(Interleukin 5) ELISA Kit

Catalogue No.

EH0200

Alias

IL-5 ELISA Kit, IL5 ELISA Kit, EDF ELISA Kit, TRF ELISA Kit, E-CSF ELISA Kit, ESGF ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

IL-5 ELISA Kit allows for the in vitro quantitative determination of IL-5 concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

15.625-1000pg/ml

Sensitivity

< 9.375pg/ml

Standard curve

Human IL-5(Interleukin 5) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of IL-5 and the recovery rates were calculated by comparing the measured value to the expected amount of IL-5 in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 94-104 99
EDTA plasma(n=5) 89-100 94
heparin plasma(n=5) 92-105 96
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-5 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 90-102% 92-105% 86-105% 86-98%
EDTA plasma(n=5) 88-98% 85-95% 84-100% 83-95%
heparin plasma(n=5) 88-98% 80-98% 84-100% 83-93%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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