Human IL-1ra/IL-1F3(Interleukin 1 Receptor Antagonist) ELISA Kit

说明书
  • 规格
Product name

Human IL-1ra/IL-1F3(Interleukin 1 Receptor Antagonist) ELISA Kit

Catalogue No.

EH0172

Alias

IL-1RA ELISA Kit, IL-1ra ELISA Kit, IL-1F3 ELISA Kit, IL1RN ELISA Kit, DIRA ELISA Kit, ICIL-1RA ELISA Kit, IL-1RN ELISA Kit, IL-1ra3 ELISA Kit, IL1F3 ELISA Kit, IL1RA ELISA Kit, IRAP ELISA Kit, MVCD4 ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

IL-1ra/IL-1F3 ELISA Kit allows for the in vitro quantitative determination of IL-1ra/IL-1F3 concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

31.25-2000pg/ml

Sensitivity

< 18.75pg/ml

Standard curve

Human IL-1ra/IL-1F3(Interleukin 1 Receptor Antagonist) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of IL-1ra/IL-1F3 and the recovery rates were calculated by comparing the measured value to the expected amount of IL-1ra/IL-1F3 in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 90-103 95
EDTA plasma(n=5) 86-102 96
heparin plasma(n=5) 86-95 91
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-1ra/IL-1F3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 89-104% 86-105% 88-96% 91-105%
EDTA plasma(n=5) 83-101% 85-100% 82-97% 87-101%
heparin plasma(n=5) 91-98% 85-98% 81-99% 87-98%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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