Human IL-1alpha(Interleukin 1 Alpha) ELISA Kit

说明书
  • 规格
Product name

Human IL-1alpha(Interleukin 1 Alpha) ELISA Kit

Catalogue No.

EH0184

Alias

IL-1α ELISA Kit, IL-1alpha ELISA Kit, IL1A ELISA Kit, IL1-A ELISA Kit, IL1 ELISA Kit, IL1F1 ELISA Kit, Preinterleukin 1 Alpha ELISA Kit, Hematopoietin-1 ELISA Kit, Pro-Interleukin-1-Alpha ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

IL-1alpha ELISA Kit allows for the in vitro quantitative determination of IL-1alpha concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

3.906-250pg/ml

Sensitivity

< 2.344pg/ml

Standard curve

Human IL-1alpha(Interleukin 1 Alpha) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of IL-1alpha and the recovery rates were calculated by comparing the measured value to the expected amount of IL-1alpha in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 86-100 95
EDTA plasma(n=5) 88-98 94
heparin plasma(n=5) 89-100 93
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-1alpha and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 90-105% 91-105% 85-98% 85-101%
EDTA plasma(n=5) 86-101% 82-98% 86-100% 82-91%
heparin plasma(n=5) 88-94% 86-98% 83-98% 88-99%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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