Human IGFBP-1(Insulin-like growth factor-binding protein 1) ELISA Kit

说明书
  • 规格
Product name

Human IGFBP-1(Insulin-like growth factor-binding protein 1) ELISA Kit

Catalogue No.

EH0167

Alias

IGFBP-1 ELISA Kit, IGFBP1 ELISA Kit, AFBP ELISA Kit, IBP1 ELISA Kit, IGF-BP25 ELISA Kit, PP12 ELISA Kit, placental protein 12 ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

IGFBP-1 ELISA Kit allows for the in vitro quantitative determination of IGFBP-1 concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

0.156-10ng/ml

Sensitivity

< 0.094ng/ml

Standard curve

Human IGFBP-1(Insulin-like growth factor-binding protein 1) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of IGFBP-1 and the recovery rates were calculated by comparing the measured value to the expected amount of IGFBP-1 in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 89-105 98
EDTA plasma(n=5) 90-102 95
heparin plasma(n=5) 86-103 95
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IGFBP-1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 88-104% 85-105% 86-104% 85-104%
EDTA plasma(n=5) 82-94% 86-101% 84-97% 83-98%
heparin plasma(n=5) 80-93% 87-98% 86-97% 93-99%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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