Human IFNA1(Interferon alpha-1/13 ) ELISA Kit

说明书
  • 规格
Product name

Human IFNA1(Interferon alpha-1/13 ) ELISA Kit

Catalogue No.

EH0502

Alias

IFNA1(Interferon alpha-1 ELISA Kit, 13 ) ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

IFNA1 ELISA Kit allows for the in vitro quantitative determination of IFNA1 concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

7.8-500pg/ml

Sensitivity

< 4.688pg/ml

Standard curve

Human IFNA1(Interferon alpha-1/13 ) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of IFNA1 and the recovery rates were calculated by comparing the measured value to the expected amount of IFNA1 in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 86-103 94
EDTA plasma(n=5) 86-105 94
heparin plasma(n=5) 88-102 97
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IFNA1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 86-99% 89-105% 85-102% 90-105%
EDTA plasma(n=5) 82-100% 83-100% 88-100% 91-101%
heparin plasma(n=5) 82-95% 83-95% 82-98% 83-98%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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