Human HCRT(Orexin) ELISA Kit

说明书
  • 规格
Product name

Human HCRT(Orexin) ELISA Kit

Catalogue No.

EH1045

Alias

HCRT ELISA Kit, OX ELISA Kit, PPORX ELISA Kit, PPOX ELISA Kit, Orexin ELISA Kit, Hypocretin ELISA Kit, Hcrt ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

HCRT ELISA Kit allows for the in vitro quantitative determination of HCRT concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

15.6-1000pg/ml

Sensitivity

< 9.375pg/ml

Standard curve

Human HCRT(Orexin) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of HCRT and the recovery rates were calculated by comparing the measured value to the expected amount of HCRT in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 90-100 94
EDTA plasma(n=5) 88-104 94
heparin plasma(n=5) 87-104 93
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of HCRT and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 87-104% 85-105% 87-101% 88-104%
EDTA plasma(n=5) 89-97% 90-99% 89-99% 84-99%
heparin plasma(n=5) 81-98% 80-94% 81-97% 82-97%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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