Human Gp130/IL6ST(Interleukin-6 receptor subunit beta) ELISA Kit

说明书
  • 规格
Product name

Human Gp130/IL6ST(Interleukin-6 receptor subunit beta) ELISA Kit

Catalogue No.

EH0154

Alias

sgp130(soluble Glucoprotein 130) ELISA Kit, IL6ST ELISA Kit, CD130 ELISA Kit, CDW130 ELISA Kit, IL-6RB ELISA Kit, IR6RB ELISA Kit, Interleukin 6 signal transducer ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

Gp130/IL6ST ELISA Kit allows for the in vitro quantitative determination of Gp130/IL6ST concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

0.25-16ng/ml

Sensitivity

< 0.15ng/ml

Standard curve

Human Gp130/IL6ST(Interleukin-6 receptor subunit beta) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of Gp130/IL6ST and the recovery rates were calculated by comparing the measured value to the expected amount of Gp130/IL6ST in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 85-104 94
EDTA plasma(n=5) 86-100 94
heparin plasma(n=5) 86-102 91
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Gp130/IL6ST and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 87-97% 85-97% 90-105% 85-103%
EDTA plasma(n=5) 82-98% 84-100% 87-99% 84-99%
heparin plasma(n=5) 80-90% 82-100% 84-99% 85-96%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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