Human G-CSF(Granulocyte Colony Stimulating Factor 3) ELISA Kit

说明书
  • 规格
Product name

Human G-CSF(Granulocyte Colony Stimulating Factor 3) ELISA Kit

Catalogue No.

EH0149

Alias

G-CSF ELISA Kit, CSF3 ELISA Kit, C17orf33 ELISA Kit, CSF3OS ELISA Kit, Pluripoietin ELISA Kit, Filgrastim ELISA Kit, Lenograstim ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

G-CSF ELISA Kit allows for the in vitro quantitative determination of G-CSF concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

39.063-2500pg/ml

Sensitivity

< 23.438pg/ml

Standard curve

Human G-CSF(Granulocyte Colony Stimulating Factor 3) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of G-CSF and the recovery rates were calculated by comparing the measured value to the expected amount of G-CSF in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 85-104 95
EDTA plasma(n=5) 88-99 93
heparin plasma(n=5) 88-102 95
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of G-CSF and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 85-97% 90-102% 99-105% 88-102%
EDTA plasma(n=5) 83-96% 89-100% 82-96% 84-96%
heparin plasma(n=5) 83-94% 80-93% 89-94% 84-98%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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