Human EGF(Epidermal Growth Factor) ELISA Kit

说明书
  • 规格
Product name

Human EGF(Epidermal Growth Factor) ELISA Kit

Catalogue No.

EH0009

Alias

EGF(Epidermal growth factor) ELISA Kit, URG ELISA Kit, HOMG4 ELISA Kit, Beta-Urogastrone ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

EGF ELISA Kit allows for the in vitro quantitative determination of EGF concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

3.906-250pg/ml

Sensitivity

< 2.344pg/ml

Standard curve

Human EGF(Epidermal Growth Factor) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of EGF and the recovery rates were calculated by comparing the measured value to the expected amount of EGF in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 86-105 94
EDTA plasma(n=5) 88-101 93
heparin plasma(n=5) 88-103 96
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of EGF and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 85-102% 88-104% 86-103% 90-105%
EDTA plasma(n=5) 85-93% 92-97% 84-100% 83-95%
heparin plasma(n=5) 81-96% 85-97% 84-100% 83-98%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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