Human DKK-3(Dickkopf-related protein 3) ELISA Kit

说明书
  • 规格
Product name

Human DKK-3(Dickkopf-related protein 3) ELISA Kit

Catalogue No.

EH0114

Alias

DKK-3 ELISA Kit, Dickkopf-3 ELISA Kit, Dkk3 ELISA Kit, REIC ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

DKK-3 ELISA Kit allows for the in vitro quantitative determination of DKK-3 concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

62.5-4000pg/ml

Sensitivity

< 37.5pg/ml

Standard curve

Human DKK-3(Dickkopf-related protein 3) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of DKK-3 and the recovery rates were calculated by comparing the measured value to the expected amount of DKK-3 in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 90-103 96
EDTA plasma(n=5) 91-103 96
heparin plasma(n=5) 89-105 97
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of DKK-3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 86-104% 87-102% 85-104% 89-98%
EDTA plasma(n=5) 84-97% 85-97% 82-100% 82-95%
heparin plasma(n=5) 92-100% 85-99% 80-98% 84-99%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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