Human DIO2(Type II iodothyronine deiodinase) ELISA Kit

说明书
  • 规格
Product name

Human DIO2(Type II iodothyronine deiodinase) ELISA Kit

Catalogue No.

EH0963

Alias

DIO2 ELISA Kit, Type-II 5′-deiodinase ELISA Kit, Type 2 DI ELISA Kit, 5DII ELISA Kit, DIOII ELISA Kit, ITDI2 ELISA Kit, TXDI2 ELISA Kit

Detection method

Competitive ELISA, Coated with Antibody

Application

DIO2 ELISA Kit allows for the in vitro quantitative determination of DIO2 concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

0.156-10ng/ml

Sensitivity

< 0.094ng/ml

Standard curve

Human DIO2(Type II iodothyronine deiodinase) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of DIO2 and the recovery rates were calculated by comparing the measured value to the expected amount of DIO2 in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 88-104 94
EDTA plasma(n=5) 87-105 99
heparin plasma(n=5) 85-100 92
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of DIO2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 85-104% 92-105% 85-93% 87-100%
EDTA plasma(n=5) 82-99% 83-99% 82-94% 83-100%
heparin plasma(n=5) 84-100% 83-95% 92-98% 83-91%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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