Human DEFB1(Beta-defensin 1) ELISA Kit

说明书
  • 规格
Product name

Human DEFB1(Beta-defensin 1) ELISA Kit

Catalogue No.

EH0868

Alias

DEFB1 ELISA Kit, Beta-defensin 1 ELISA Kit, BD1 ELISA Kit, HBD1 ELISA Kit, BD-1 ELISA Kit, hBD-1 ELISA Kit, Defensin Beta 1 ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

DEFB1 ELISA Kit allows for the in vitro quantitative determination of DEFB1 concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

31.25-2000pg/ml

Sensitivity

< 18.75pg/ml

Standard curve

Human DEFB1(Beta-defensin 1) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of DEFB1 and the recovery rates were calculated by comparing the measured value to the expected amount of DEFB1 in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 88-101 95
EDTA plasma(n=5) 97-103 100
heparin plasma(n=5) 95-104 99
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of DEFB1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 90-98% 86-104% 85-100% 87-105%
EDTA plasma(n=5) 87-96% 82-101% 89-100% 83-99%
heparin plasma(n=5) 88-97% 80-91% 90-100% 83-91%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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