Human CYP27B1(25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial) ELISA Kit

说明书
  • 规格
Product name

Human CYP27B1(25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial) ELISA Kit

Catalogue No.

EH1158

Alias

CYP27B1 ELISA Kit, VD3 1A hydroxylase ELISA Kit, Cytochrome P450VD1-alpha ELISA Kit, Cytochrome P450C1 alpha ELISA Kit, Cytochrome P450 subfamily XXVIIB polypeptide 1 ELISA Kit, Calcidiol 1-monooxygenase ELISA Kit, Cytochrome p450 27B1 ELISA Kit, 25-OHD-1 alpha-hydroxylase ELISA Kit, 25-hydroxyvitamin D(3) 1-alpha-hydroxylase ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

CYP27B1 ELISA Kit allows for the in vitro quantitative determination of CYP27B1 concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

0.78-50ng/ml

Sensitivity

< 0.469ng/ml

Standard curve

Human CYP27B1(25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of CYP27B1 and the recovery rates were calculated by comparing the measured value to the expected amount of CYP27B1 in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 86-104 92
EDTA plasma(n=5) 85-104 95
heparin plasma(n=5) 90-105 98
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CYP27B1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 98-104% 91-102% 87-102% 93-102%
EDTA plasma(n=5) 85-92% 87-101% 82-98% 82-100%
heparin plasma(n=5) 83-99% 86-100% 84-99% 86-92%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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