Human CYFRA21-1(Cytokeratin Fragment Antigen 21-1) ELISA Kit

说明书
  • 规格
Product name

Human CYFRA21-1(Cytokeratin Fragment Antigen 21-1) ELISA Kit

Catalogue No.

EH0364

Alias

CYFRA21-1(Cytokeratin Fragment Antigen 21-1) ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

CYFRA21-1 ELISA Kit allows for the in vitro quantitative determination of CYFRA21-1 concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

0.313-20ng/ml

Sensitivity

< 0.188ng/ml

Standard curve

Human CYFRA21-1(Cytokeratin Fragment Antigen 21-1) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of CYFRA21-1 and the recovery rates were calculated by comparing the measured value to the expected amount of CYFRA21-1 in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 86-99 94
EDTA plasma(n=5) 85-100 92
heparin plasma(n=5) 85-105 96
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CYFRA21-1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 88-103% 90-105% 87-98% 94-105%
EDTA plasma(n=5) 82-100% 88-101% 83-94% 82-101%
heparin plasma(n=5) 84-99% 82-94% 83-93% 81-88%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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