Human CNTF(Ciliary neurotrophic factor ) ELISA Kit

说明书
  • 规格
Product name

Human CNTF(Ciliary neurotrophic factor ) ELISA Kit

Catalogue No.

EH0497

Alias

CNTF ELISA Kit, HCNTF ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

CNTF ELISA Kit allows for the in vitro quantitative determination of CNTF concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

31.25-2000pg/ml

Sensitivity

< 18.75pg/ml

Standard curve

Human CNTF(Ciliary neurotrophic factor ) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of CNTF and the recovery rates were calculated by comparing the measured value to the expected amount of CNTF in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 86-102 97
EDTA plasma(n=5) 94-100 96
heparin plasma(n=5) 87-104 94
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CNTF and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 87-101% 87-103% 90-99% 86-100%
EDTA plasma(n=5) 82-93% 82-100% 82-88% 82-98%
heparin plasma(n=5) 82-97% 80-92% 84-96% 80-95%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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