Human CD40L(Cluster of Differentiation 40 Ligand) ELISA Kit

说明书
  • 规格
Product name

Human CD40L(Cluster of Differentiation 40 Ligand) ELISA Kit

Catalogue No.

EH0086

Alias

CD40L ELISA Kit, TNFSF5 ELISA Kit, TNFSF5,CD40LG ELISA Kit, CD154 ELISA Kit, HIGM1 ELISA Kit, IGM ELISA Kit, IMD3 ELISA Kit, T-BAM ELISA Kit, TRAP ELISA Kit, Gp39 ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

CD40L ELISA Kit allows for the in vitro quantitative determination of CD40L concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

62.5-4000pg/ml

Sensitivity

< 37.5pg/ml

Standard curve

Human CD40L(Cluster of Differentiation 40 Ligand) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of CD40L and the recovery rates were calculated by comparing the measured value to the expected amount of CD40L in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 89-104 94
EDTA plasma(n=5) 88-103 96
heparin plasma(n=5) 89-100 95
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CD40L and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 93-102% 86-105% 88-105% 88-105%
EDTA plasma(n=5) 85-95% 84-98% 82-100% 82-99%
heparin plasma(n=5) 91-98% 80-93% 80-99% 80-98%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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