Human CCL11(Eotaxin) ELISA Kit

说明书
  • 规格
Product name

Human CCL11(Eotaxin) ELISA Kit

Catalogue No.

EH0121

Alias

CCL11 ELISA Kit, ECF ELISA Kit, Eotaxin ELISA Kit, chemokine ligand 11 ELISA Kit, Eotaxin-1 ELISA Kit, Eosinophil chemotactic protein ELISA Kit, SCYA11Small-inducible cytokine A11 ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

CCL11 ELISA Kit allows for the in vitro quantitative determination of CCL11 concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

15.6-1000pg/ml

Sensitivity

< 9.375pg/ml

Standard curve

Human CCL11(Eotaxin) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of CCL11 and the recovery rates were calculated by comparing the measured value to the expected amount of CCL11 in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 93-99 97
EDTA plasma(n=5) 86-100 93
heparin plasma(n=5) 87-105 99
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CCL11 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 88-100% 90-104% 86-93% 85-89%
EDTA plasma(n=5) 90-100% 83-96% 83-93% 86-100%
heparin plasma(n=5) 80-98% 84-92% 80-94% 83-92%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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