Human C5(Complement C5) ELISA Kit

说明书
  • 规格
Product name

Human C5(Complement C5) ELISA Kit

Catalogue No.

EH0680

Alias

C5(Complement Component 5) ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

C5 ELISA Kit allows for the in vitro quantitative determination of C5 concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

0.781-50ng/ml

Sensitivity

< 0.469ng/ml

Standard curve

Human C5(Complement C5) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of C5 and the recovery rates were calculated by comparing the measured value to the expected amount of C5 in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 91-103 95
EDTA plasma(n=5) 86-103 96
heparin plasma(n=5) 85-100 92
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of C5 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 86-104% 85-103% 87-104% 87-104%
EDTA plasma(n=5) 83-94% 83-86% 93-100% 83-99%
heparin plasma(n=5) 84-99% 80-100% 88-97% 81-97%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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