Human BID(BH3-interacting domain death agonist) ELISA Kit

说明书
  • 规格
Product name

Human BID(BH3-interacting domain death agonist) ELISA Kit

Catalogue No.

EH0983

Alias

BID(BH3-interacting domain death agonist) ELISA Kit, apoptic death agonist ELISA Kit, BH3 interacting domain death agonist ELISA Kit, BID isoform ES(1b) ELISA Kit, BID isoform L(2) ELISA Kit, BID isoform Si6 ELISA Kit, desmocollin type 4 ELISA Kit, FP497 ELISA Kit, Human BID coding sequence ELISA Kit, p22 BID ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

BID ELISA Kit allows for the in vitro quantitative determination of BID concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

0.313-20ng/ml

Sensitivity

< 0.188ng/ml

Standard curve

Human BID(BH3-interacting domain death agonist) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of BID and the recovery rates were calculated by comparing the measured value to the expected amount of BID in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 88-104 98
EDTA plasma(n=5) 91-104 95
heparin plasma(n=5) 90-105 98
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of BID and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 85-103% 86-103% 87-102% 87-104%
EDTA plasma(n=5) 87-100% 84-98% 86-98% 83-99%
heparin plasma(n=5) 81-90% 82-96% 80-99% 80-100%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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