Human BECN1(Beclin-1) ELISA Kit

说明书
  • 规格
Product name

Human BECN1(Beclin-1) ELISA Kit

Catalogue No.

EH1146

Alias

BECN1(Beclin 1) ELISA Kit, ATG6 ELISA Kit, GT197 ELISA Kit, VPS30 ELISA Kit, ATG6 autophagy related 6 homolog ELISA Kit, beclin 1, autophagy related ELISA Kit, beclin-1 ELISA Kit, Coiled-coil myosin-like BCL2-interacting protein ELISA Kit, Protein GT197 ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

BECN1 ELISA Kit allows for the in vitro quantitative determination of BECN1 concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

0.156-10ng/ml

Sensitivity

< 0.094ng/ml

Standard curve

Human BECN1(Beclin-1) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of BECN1 and the recovery rates were calculated by comparing the measured value to the expected amount of BECN1 in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 88-99 93
EDTA plasma(n=5) 93-104 98
heparin plasma(n=5) 86-99 93
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of BECN1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 88-102% 95-102% 93-98% 85-105%
EDTA plasma(n=5) 84-96% 84-100% 85-99% 84-101%
heparin plasma(n=5) 81-100% 84-98% 86-97% 85-96%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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