Human APOB(Apolipoprotein B-100) ELISA Kit

说明书
  • 规格
Product name

Human APOB(Apolipoprotein B-100) ELISA Kit

Catalogue No.

EH0620

Alias

ApoB(Apolipoprotein B) ELISA Kit, APOB ELISA Kit, Apo-B ELISA Kit, FLDB ELISA Kit, LDLCQ4 ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

APOB ELISA Kit allows for the in vitro quantitative determination of APOB concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

78.125-5000ng/ml

Sensitivity

< 46.875ng/ml

Standard curve

Human APOB(Apolipoprotein B-100) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of APOB and the recovery rates were calculated by comparing the measured value to the expected amount of APOB in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 91-103 99
EDTA plasma(n=5) 85-104 96
heparin plasma(n=5) 92-103 97
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of APOB and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 85-105% 87-101% 85-99% 86-104%
EDTA plasma(n=5) 92-100% 84-90% 87-97% 86-96%
heparin plasma(n=5) 82-90% 84-93% 80-97% 85-95%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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