Human AKT1(RAC-alpha serine/threonine-protein kinase ) ELISA Kit

说明书
  • 规格
Product name

Human AKT1(RAC-alpha serine/threonine-protein kinase ) ELISA Kit

Catalogue No.

EH0532

Alias

PKB ELISA Kit, AKT1 ELISA Kit, PKB alpha ELISA Kit, PRKBA ELISA Kit, RAC-alpha ELISA Kit, AKT ELISA Kit, PKBMGC99656 ELISA Kit, Protein kinase B ELISA Kit, Proto-oncogene c-Akt ELISA Kit, rac protein kinase alpha ELISA Kit, RAC-ALPHA ELISA Kit, RAC-alpha serine ELISA Kit, threonine-protein kinase ELISA Kit, RAC-PK-alpha ELISA Kit, RACPKB-ALPHA ELISA Kit, v-akt murine thymoma viral oncogene homolog 1 ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

AKT1 ELISA Kit allows for the in vitro quantitative determination of AKT1 concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

0.156-10ng/ml

Sensitivity

< 0.094ng/ml

Standard curve

Human AKT1(RAC-alpha serine/threonine-protein kinase ) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of AKT1 and the recovery rates were calculated by comparing the measured value to the expected amount of AKT1 in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 86-105 94
EDTA plasma(n=5) 85-102 95
heparin plasma(n=5) 90-104 97
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of AKT1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 86-93% 91-104% 85-97% 93-104%
EDTA plasma(n=5) 84-98% 83-96% 89-95% 87-94%
heparin plasma(n=5) 81-93% 83-89% 89-100% 84-93%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

服务

点击这里给我发消息

热线

027-87384275