Human AGRP(Agouti-related protein ) ELISA Kit

说明书
  • 规格
Product name

Human AGRP(Agouti-related protein ) ELISA Kit

Catalogue No.

EH0587

Alias

AGRP(Agouti-related protein) ELISA Kit, AGRT ELISA Kit, ASIP2 ELISA Kit, ART ELISA Kit, agouti-related protein ELISA Kit, Agrt ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

AGRP ELISA Kit allows for the in vitro quantitative determination of AGRP concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

31.25-2000pg/ml

Sensitivity

< 18.75pg/ml

Standard curve

Human AGRP(Agouti-related protein ) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of AGRP and the recovery rates were calculated by comparing the measured value to the expected amount of AGRP in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 92-103 98
EDTA plasma(n=5) 87-100 95
heparin plasma(n=5) 89-100 94
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of AGRP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 86-105% 87-105% 91-103% 89-99%
EDTA plasma(n=5) 83-101% 95-100% 88-99% 83-98%
heparin plasma(n=5) 84-100% 82-99% 80-96% 87-100%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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