Human ACA-IgG (Anti-Cardiolipin Antibody IgG) ELISA Kit

说明书
  • 规格
Product name

Human ACA-IgG (Anti-Cardiolipin Antibody IgG) ELISA Kit

Catalogue No.

EH0478

Alias

ACA-IgG(Anti-Cardiolipin Antibody IgG) ELISA Kit

Detection method

Sandwich ELISA, Double Antigen

Application

ACA-IgG ELISA Kit allows for the in vitro quantitative determination of ACA-IgG concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

5-320ug/ml

Sensitivity

< 3μg/ml

Standard curve

Human ACA-IgG (Anti-Cardiolipin Antibody IgG) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of ACA-IgG and the recovery rates were calculated by comparing the measured value to the expected amount of ACA-IgG in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 86-101 96
EDTA plasma(n=5) 87-98 92
heparin plasma(n=5) 88-99 93
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACA-IgG and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 86-93% 88-102% 93-104% 88-100%
EDTA plasma(n=5) 83-100% 83-99% 82-98% 82-94%
heparin plasma(n=5) 80-96% 85-99% 87-95% 81-96%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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