Human 5-NT (5-Nucleotidase) ELISA Kit

说明书
  • 规格
Product name

Human 5-NT (5-Nucleotidase) ELISA Kit

Catalogue No.

EH0468

Alias

5-NT ELISA Kit, NT5E ELISA Kit, 5′-NT ELISA Kit, CD73 ELISA Kit, E5NT ELISA Kit, eNT ELISA Kit, NTE ELISA Kit, eN ELISA Kit, NT ELISA Kit, NT5 ELISA Kit, 5′ nucleotidase(CD73) ELISA Kit, CD73 antigen ELISA Kit, Ecto-5′-nucleotidase ELISA Kit, 5′-nucleotidase ELISA Kit, Purine 5-Prime-Nucleotidase ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

5-NT ELISA Kit allows for the in vitro quantitative determination of 5-NT concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

0.313-20ng/ml

Sensitivity

< 0.188ng/ml

Standard curve

Human 5-NT (5-Nucleotidase) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of 5-NT and the recovery rates were calculated by comparing the measured value to the expected amount of 5-NT in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 85-102 95
EDTA plasma(n=5) 91-104 98
heparin plasma(n=5) 91-97 94
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 5-NT and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 86-103% 86-104% 85-99% 95-105%
EDTA plasma(n=5) 84-101% 85-101% 86-101% 89-101%
heparin plasma(n=5) 81-97% 83-92% 80-95% 80-98%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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