Human 15-LO (Arachidonate 15-Lipoxygenase) ELISA Kit

说明书
  • 规格
Product name

Human 15-LO (Arachidonate 15-Lipoxygenase) ELISA Kit

Catalogue No.

EH0204

Alias

15-LO ELISA Kit, ALOX15 ELISA Kit, 15-LOX-1 ELISA Kit, 15LOX-1 ELISA Kit, LOX15 ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

15-LO ELISA Kit allows for the in vitro quantitative determination of 15-LO concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

62.5-4000pg/ml

Sensitivity

< 37.5pg/ml

Standard curve

Human 15-LO (Arachidonate 15-Lipoxygenase) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of 15-LO and the recovery rates were calculated by comparing the measured value to the expected amount of 15-LO in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 87-97 94
EDTA plasma(n=5) 85-101 95
heparin plasma(n=5) 85-99 90
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 15-LO and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 89-104% 86-101% 85-96% 87-101%
EDTA plasma(n=5) 88-95% 82-100% 84-101% 88-100%
heparin plasma(n=5) 81-99% 81-90% 88-97% 92-99%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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