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Q&A for Possible reasons of contaminated

Time: 2017-05-15 17:08:14

Q&A for Possible reasons of contaminated

 

1.
Wash buffer was contaminated?

Cross contamination with other reagents?
Well plate insufficiently shake after wash
plate?

 
2.
Dilution buffer was contaminated?

Clean and colorless on reagents when it is
in preparation?

 
3.
Contaminated on incubation

Use new plate sealer when it is on
incubated?

Any liquid or dirty on well plate?
 
4. TMB
Substrate Solution was contaminated

Any change on the color of TMB?
Substrate incubation carried out in light?
 
5.
Pipette was contaminated?

Cross-used when it is in test?
 
6.
Reagents preparation

Correct and proper dilution rate for all
reagents?

 
7.
Keep the well plate completely dry at any time? 


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