Rat IL-6(Interleukin-6) ELISA Kit

Manual
  • Specifications
Product name

Rat IL-6(Interleukin-6) ELISA Kit

Catalogue No.

ER0042

Detection method

Sandwich ELISA, Double Antibody

Application

IL-6 ELISA Kit allows for the in vitro quantitative determination of IL-6 concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

62.5-4000pg/ml

Sensitivity

< 37.5pg/ml

Standard curve

Rat IL-6(Interleukin-6) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of IL-6 and the recovery rates were calculated by comparing the measured value to the expected amount of IL-6 in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 91-97 95
EDTA plasma(n=5) 85-105 95
heparin plasma(n=5) 89-103 95
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-6 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 88-104% 85-105% 92-103% 90-105%
EDTA plasma(n=5) 82-100% 83-99% 83-100% 83-99%
heparin plasma(n=5) 80-100% 84-96% 82-96% 85-98%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

Service

点击这里给我发消息

Hotline

027-87384275