Products

Rabbit OVA IgA(Ovalbumin IgA) ELISA Kit

Alias:OVA IgA ELISA Kit, Ovalbumin IgA ELISA Kit
Catalogue No.:ERB0152Species:Rabbit
Range:0.391-25ng/mlSensitivity:0.234ng/ml
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Product Name
Rabbit OVA IgA(Ovalbumin IgA) ELISA Kit
Alias
OVA IgA ELISA Kit, Ovalbumin IgA ELISA Kit
Catalogue No.
ERB0152
Size
48T/96T
Species
Rabbit
Sample Type
Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
Detection Method
Capture ELISA
Detection Wavelength
OD450
Reaction Duration
3 hours
Range
0.391-25ng/ml
Sensitivity
0.234ng/ml
Storage
2-8°C(Sealed), Don't cryopreserve.
Specificity
Specifically binds with OVA IgA , no obvious cross reaction with other analogues.
ELISA Kit Components
Kit Components Item Size(48T) Size(96T) Storage Condition for Opened Kit
E001 ELISA Microplate(Dismountable) 8×6 8×12 Put the rest strips into a sealed foil bag with the desiccant. Stored for 1 month at 2-8°C; Stored for 6 month at -20°C
E002 Lyophilized Standard 1vial 2vial Put the rest standards into a desiccant bag. Stored for 1 month at 2-8°C; Stored for 6 month at -20°C
E003 HRP-Antigen(Concentrated, 100X) 60ul 120ul 2-8°C (Avoid Direct Light)
E024 TMB Substrate 5ml 10ml
E039 Sample Dilution Buffer 10ml 20ml 2-8°C
E040 Antigen Dilution Buffer 5ml 10ml
E026 Stop Solution 5ml 10ml
E038 Wash Buffer(Concentrated, 25X) 15ml 30ml
E006 Plate Sealer 3 pieces 5 pieces  
E007 Product Description 1 copy 1 copy
Required Instruments and Reagents
  1. Microplate reader (wavelength: 450nm)
  2. 37°C incubator (CO2 incubator for cell culture is not recommenced.)
  3. Automated plate washer or multi-channel pipette/5ml pipettor (for manual washing purpose)
  4. Precision single (0.5-10μL, 5-50μL, 20-200μL, 200-1000μL) and multi-channel pipette with disposable tips(Calibration is required before use.)
  5. Sterile tubes and Eppendorf tubes with disposable tips
  6. Absorbent paper and loading slot
  7. Deionized or distilled water
Standard Curve

This product is detected by QC department and meets performance required in the manual. (Laboratory Humidity: 20%-60%; Temperature: 18°C -25°C; Equilibrate TMB substrate to 37°C before staining. After adding into the ELISA wells, incubate for 15min at 37°C in dark.)

Due to different assay environments and operations, assay data below and standard curve are provided for reference. Experimenters should establish standard curve according to their own assay.

STD.(ng/ml) OD-1 OD-2 Average
0 0.107 0.111 0.109
0.391 0.137 0.141 0.139
0.781 0.168 0.172 0.17
1.562 0.246 0.254 0.25
3.125 0.597 0.615 0.606
6.25 0.954 0.982 0.968
12.5 1.619 1.665 1.642
25 2.159 2.221 2.19
Recovery

Add a certain amount of OVA IgA into the sample. Calculate the recovery by comparing the measured value with the expected amount of OVA IgA in the sample.

Sample Type Recovery Range(%) Average(%)
serum(n=10) 90-103 96
EDTA plasma(n=10) 85-101 90
Heparin plasma(n=10) 89-102 94
Linearity

Dilute the sample with a certain amount of OVA IgA at 1:2, 1:4 and 1:8 to get the recovery range.

Sample Type 1:2 1:4 1:8
serum(n=10) 86-103% 85-101% 89-95%
EDTA plasma(n=10) 85-97% 86-94% 85-98%
Heparin plasma(n=10) 87-98% 92-100% 89-97%
Precision(%)

Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on the same plate.

Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.

Item Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/ml) 0.86 3.23 12.15 0.92 3.43 12.64
Standard deviation 0.04 0.18 0.65 0.05 0.19 0.69
CV(%) 4.86 5.43 5.32 5.26 5.46 5.46
Stability

Perform the stability test for the sealed kit at 37°C and 2-8°C and get relevant data.

ELISA kit(n=5) 37°C for 1 month 2-8°C for 6 months
Average(%) 80 95-100