Human VCAM-1(Vascular cell adhesion protein 1) ELISA Kit

Manual
  • Specifications
Product name

Human VCAM-1(Vascular cell adhesion protein 1) ELISA Kit

Catalogue No.

EH0326

Alias

VCAM-1 ELISA Kit, CD106 ELISA Kit, sVCAM-1 ELISA Kit, CD106 antigen ELISA Kit, INCAM-100 ELISA Kit, L1CAM ELISA Kit, vascular cell adhesion molecule 1 ELISA Kit, vascular cell adhesion protein 1 ELISA Kit, V-CAM 1 ELISA Kit, VCAM1 ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

VCAM-1 ELISA Kit allows for the in vitro quantitative determination of VCAM-1 concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

0.156-10ng/ml

Sensitivity

< 0.094ng/ml

Standard curve

Human VCAM-1(Vascular cell adhesion protein 1) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of VCAM-1 and the recovery rates were calculated by comparing the measured value to the expected amount of VCAM-1 in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 85-103 93
EDTA plasma(n=5) 89-104 96
heparin plasma(n=5) 96-103 100
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of VCAM-1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 86-105% 85-99% 86-104% 89-103%
EDTA plasma(n=5) 83-100% 85-89% 83-96% 96-100%
heparin plasma(n=5) 86-98% 82-98% 80-98% 80-99%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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