Human TRAIL/TNFSF18(Tumor necrosis factor ligand superfamily member 18) ELISA Kit

Manual
  • Specifications
Product name

Human TRAIL/TNFSF18(Tumor necrosis factor ligand superfamily member 18) ELISA Kit

Catalogue No.

EH0320

Alias

TNFSF18 ELISA Kit, GITRL ELISA Kit, GITR Ligand ELISA Kit, AITRL ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

TRAIL/TNFSF18 ELISA Kit allows for the in vitro quantitative determination of TRAIL/TNFSF18 concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

0.156-10ng/ml

Sensitivity

< 0.094ng/ml

Standard curve

Human TRAIL/TNFSF18(Tumor necrosis factor ligand superfamily member 18) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of TRAIL/TNFSF18 and the recovery rates were calculated by comparing the measured value to the expected amount of TRAIL/TNFSF18 in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 86-104 97
EDTA plasma(n=5) 89-105 98
heparin plasma(n=5) 86-103 96
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TRAIL/TNFSF18 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 85-100% 87-95% 87-103% 94-105%
EDTA plasma(n=5) 86-99% 84-92% 85-101% 88-99%
heparin plasma(n=5) 83-97% 80-86% 80-97% 88-94%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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