Human TPMT(Thiopurine S-methyltransferase) ELISA Kit

Manual
  • Specifications
Product name

Human TPMT(Thiopurine S-methyltransferase) ELISA Kit

Catalogue No.

EH1175

Alias

TPMT(Thiopurine S-methyltransferase) ELISA Kit, Thiopurine methyltransferase ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

TPMT ELISA Kit allows for the in vitro quantitative determination of TPMT concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

3.125-200ng/ml

Sensitivity

< 1.875ng/ml

Standard curve

Human TPMT(Thiopurine S-methyltransferase) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of TPMT and the recovery rates were calculated by comparing the measured value to the expected amount of TPMT in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 85-98 93
EDTA plasma(n=5) 95-100 98
heparin plasma(n=5) 87-105 96
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TPMT and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 86-104% 88-103% 85-98% 85-94%
EDTA plasma(n=5) 83-101% 84-100% 82-88% 83-101%
heparin plasma(n=5) 81-96% 81-91% 80-94% 81-100%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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