Human TNFSF10(Tumor Necrosis Factor Related Apoptosis Inducing Ligand) ELISA Kit

Manual
  • Specifications
Product name

Human TNFSF10(Tumor Necrosis Factor Related Apoptosis Inducing Ligand) ELISA Kit

Catalogue No.

EH0521

Alias

TRAIL ELISA Kit, TNFSF10 ELISA Kit, CD253 ELISA Kit, Apo-2 ligand ELISA Kit, APO2L ELISA Kit, Apo-2Ltumor necrosis factor(ligand) family, member 10 ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

TNFSF10 ELISA Kit allows for the in vitro quantitative determination of TNFSF10 concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

15.625-1000pg/ml

Sensitivity

< 9.375pg/ml

Standard curve

Human TNFSF10(Tumor Necrosis Factor Related Apoptosis Inducing Ligand) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of TNFSF10 and the recovery rates were calculated by comparing the measured value to the expected amount of TNFSF10 in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 85-98 92
EDTA plasma(n=5) 90-105 96
heparin plasma(n=5) 85-101 94
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TNFSF10 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 85-104% 85-104% 94-105% 86-105%
EDTA plasma(n=5) 88-99% 88-91% 82-89% 83-101%
heparin plasma(n=5) 80-92% 83-100% 80-88% 87-91%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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