Human TNFβ(Tumor Necrosis Factor Beta) ELISA Kit

Manual
  • Specifications
Product name

Human TNFβ(Tumor Necrosis Factor Beta) ELISA Kit

Catalogue No.

EH0303

Alias

TNFβ(Tumor Necrosis Factor Beta) ELISA Kit, TNF-β ELISA Kit, LTA ELISA Kit, Lymphotoxin alpha ELISA Kit, tnfb ELISA Kit, TNF-beta ELISA Kit, TNFSF1 ELISA Kit, TNFSF1B ELISA Kit, LT ELISA Kit, LT-alpha ELISA Kit, lymphotoxin alpha(TNF superfamily, member 1) ELISA Kit, TNFSF1TNF-beta ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

TNFβ ELISA Kit allows for the in vitro quantitative determination of TNFβ concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

15.625-1000pg/ml

Sensitivity

< 9.375pg/ml

Standard curve

Human TNFβ(Tumor Necrosis Factor Beta) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of TNFβ and the recovery rates were calculated by comparing the measured value to the expected amount of TNFβ in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 94-104 97
EDTA plasma(n=5) 86-105 93
heparin plasma(n=5) 90-100 94
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TNFβ and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 86-91% 85-99% 87-103% 88-102%
EDTA plasma(n=5) 86-100% 84-101% 82-99% 84-100%
heparin plasma(n=5) 87-100% 80-96% 81-99% 80-100%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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