Human STAT5A(Signal transducer and activator of transcription 5A) ELISA Kit

Manual
  • Specifications
Product name

Human STAT5A(Signal transducer and activator of transcription 5A) ELISA Kit

Catalogue No.

EH0601

Alias

STAT5A(Signal Transducer and Activator of Transcription 5A) ELISA Kit, MGF ELISA Kit, STAT5 ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

STAT5A ELISA Kit allows for the in vitro quantitative determination of STAT5A concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

0.625-40ng/ml

Sensitivity

< 0.375ng/ml

Standard curve

Human STAT5A(Signal transducer and activator of transcription 5A) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of STAT5A and the recovery rates were calculated by comparing the measured value to the expected amount of STAT5A in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 86-99 93
EDTA plasma(n=5) 86-103 96
heparin plasma(n=5) 87-104 97
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of STAT5A and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 92-104% 89-104% 88-101% 87-100%
EDTA plasma(n=5) 86-101% 87-100% 89-101% 90-99%
heparin plasma(n=5) 88-99% 80-100% 87-100% 84-98%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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