Human SGPP1(Sphingosine-1-phosphate phosphatase 1) ELISA Kit

Manual
  • Specifications
Product name

Human SGPP1(Sphingosine-1-phosphate phosphatase 1) ELISA Kit

Catalogue No.

EH1275

Alias

SGPP1 ELISA Kit, Sphingosine-1-phosphate phosphatase 1 ELISA Kit, SPPase1 ELISA Kit, Spp1 ELISA Kit, hSPP1 ELISA Kit, hSPPase1 ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

SGPP1 ELISA Kit allows for the in vitro quantitative determination of SGPP1 concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

78-5000pg/ml

Sensitivity

< 46.9pg/ml

Standard curve

Human SGPP1(Sphingosine-1-phosphate phosphatase 1) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of SGPP1 and the recovery rates were calculated by comparing the measured value to the expected amount of SGPP1 in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 90-103 96
EDTA plasma(n=5) 96-103 99
heparin plasma(n=5) 87-105 97
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of SGPP1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 86-102% 92-105% 86-100% 85-94%
EDTA plasma(n=5) 83-100% 82-91% 82-101% 84-90%
heparin plasma(n=5) 83-97% 81-100% 82-98% 81-100%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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