Human SERPINB4(Serpin B4) ELISA Kit

Manual
  • Specifications
Product name

Human SERPINB4(Serpin B4) ELISA Kit

Catalogue No.

EH0733

Alias

SERPINB4 ELISA Kit, PI11 ELISA Kit, SCCA2 ELISA Kit, Serpin B4 ELISA Kit, Leupin ELISA Kit, Peptidase inhibitor 11 ELISA Kit, PI-11 ELISA Kit, Squamous cell carcinoma antigen 2 ELISA Kit, SCCA-2 ELISA Kit

Detection method

Sandwich ELISA, Double Antibody

Application

SERPINB4 ELISA Kit allows for the in vitro quantitative determination of SERPINB4 concentrations in plasma, tissue homogenates and other biological fluids.

Size

96T

Range

0.156-10ng/ml

Sensitivity

< 0.094ng/ml

Standard curve

Human SERPINB4(Serpin B4) ELISA Kit
Storage

4 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of SERPINB4 and the recovery rates were calculated by comparing the measured value to the expected amount of SERPINB4 in samples.

Matrix Recovery range(%) Average(%)
serum(n=5) 86-100 94
EDTA plasma(n=5) 88-104 95
heparin plasma(n=5) 86-97 93
Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of SERPINB4 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 85-104% 88-103% 89-96% 86-102%
EDTA plasma(n=5) 82-89% 82-96% 85-98% 82-97%
heparin plasma(n=5) 80-98% 81-100% 82-100% 83-99%
CV(%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

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